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To determine physical activity levels of students in Istanbul University who take physical education (PE) courses and who don’t take PE courses a total of 110 students, 67 male and 43 female were taken as test subjects. The average age of the students were 21.4±1.940 years, average body weight was 63.336±9.851 kg. And average height was 172.85±0.83 cm. To determine the physical activity levels (PAL) of the subjects a survey, consisting of eight questions, was used and physical activity score (PAS) were calculated according to the survey. As a result of these calculations, the average PAS of all subjects were found as 62.609±21.887. In respect of the top score (139) of the survey, the average score of the subjects were 45.042±15.746%. The average score of female subjects were 52.186±19.025, and this is 37.544±13.687%. The average PAS of male subjects were 69.298±21.086 and this is determined as 49.855±15.170%. The subjects who took gym courses when studying in the university had an average PAS of 61.035±23.146. The percentage of this score was 43.909±16.652. PAS average of the students who had not took PE classes was 64.843±20.382. The percentage of this score was 46.649±14.663 as a result of the statistical analysis, it is seen that there is no difference in physical activity scores of the students who took PE classes and who did not took PE classes. This result shows us the existing physical education classes have to be re-considered.